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Promega hd fugene transfection reagent
Hd Fugene Transfection Reagent, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fugene%C2%AE+6+transfection+reagent/fugene+hd/pmc12264612-109-14-18
Average 90 stars, based on 1 article reviews
hd fugene transfection reagent - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Enabling next-generation engineered TCR-T therapies based on high-throughput TCR discovery from diagnostic tumor biopsies.
Article Snippet: 3E6 293 Vec-Baev (Biovec Pharma) or 1.2E6 Phoenix-Ampho (ATCC) cells were seeded into 10 cm2 dishes and were transfected 24 h later with 10μg plasmid DNA using serum-free DMEM and FuGENE® 6 transfection reagent (Promega).

Article Title: CD40 Ligand Potentiates Immunogenecity of Mycoplasma pneumoniae Subunit Vaccine Candidate in a Murine Model.
Article Snippet: When the cell confluence reached 70–80%, recombinant bacmid was transfected into Sf9 cells according to the instructions for FuGENE® 6 Transfection Reagent (Promega, Madison, WI, USA).

Article Title: Enabling next-generation engineered TCR-T therapies based on high-throughput TCR discovery from diagnostic tumor biopsies
Article Snippet: 3E6 293 Vec-Baev (Biovec Pharma) or 1.2E6 Phoenix-Ampho (ATCC) cells were seeded into 10 cm 2 dishes and were transfected 24 h later with 10 μg plasmid DNA using serum-free DMEM and FuGENE® 6 transfection reagent (Promega).

Article Title: A chromatin-focused CRISPR screen identifies USP22 as a barrier to somatic cell reprogramming
Article Snippet: FuGENE® 6 Transfection Reagent (Promega) used as a transfection reagent and applied according to the manufacturer’s instructions.

Article Title: Histone acetyltransferase BmMOF inhibits the proliferation of the Bombyx mori nucleopolyhedrovirus by targeting Bmp53.
Article Snippet: Bombyx mori nucleopolyhedrovirus (BmNPV) infection is a critical disease in silkworms (Bombyx mori), yet the molecular mechanism underlying the defense of Bombyx mori against BmNPV infection remains elusive.. The histone acetyltransferase MOF plays a crucial role in cellular stress response and apoptosis.. Nevertheless, its function in Bombyx mori is yet to be fully elucidated.

Article Title: Supporting Information for MARK2 phosphorylates KIF13A at a 14-3-3 binding site to polarize vesicular transport of transferrin receptor within dendrites
Article Snippet: In brief, pEGFP-N1 and lentiCRISPRv2-gRNA plasmids of MARK2 and were transfected into Neuro2A cells with FuGENE® 6 Transfection Reagent (Promega, #E2693, Madison, WI, USA) for 24h, then treated with puromycin (3μg/mL) after fed fresh medium and replaced medium in 24-48h. and treated with puromycin until more than 90% cells were expressed GFP tag.

Article Title: LncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression.
Article Snippet: LncPrep + 96kb is a long non‐coding RNA expressed in murine granulosa cells.. The 2.2‐kb fragment of lncPrep + 96kb inhibits aromatase expression and estrogen secretion in ovarian granulosa cells.. In the present study, lncPrep + 96kb‐knockout (KO) mice were generated, and significant ovarian fibrosis and reduced female fertility through fertility monitoring and superovulation.

Virus:

Article Title: Production of recombinant coiled coil silk proteins for materials synthesis.
Article Snippet: .. Virus generation and amplification for insect cell expression Passage1 (P1) virus was generated by transfecting Sf9 cells in a 6-well plate with 1 μg bacmid using FuGENE 6 (Promega) transfection reagent, following manufacturer’s instructions. .. Briefly, SF900-II suspension-adapted Sf9 in log growth phase were plated at a cell density of 1 × 106 cells/well in a 2 mL volume and allowed to settle 1 h. For each construct to be transfected, 4 ul FuGENE 6 was added to 100 μl Sf900-II serum free media (SFM) (Thermo Fisher Scientific) and incubated 5 minutes at room temperature.

Amplification:

Article Title: Production of recombinant coiled coil silk proteins for materials synthesis.
Article Snippet: .. Virus generation and amplification for insect cell expression Passage1 (P1) virus was generated by transfecting Sf9 cells in a 6-well plate with 1 μg bacmid using FuGENE 6 (Promega) transfection reagent, following manufacturer’s instructions. .. Briefly, SF900-II suspension-adapted Sf9 in log growth phase were plated at a cell density of 1 × 106 cells/well in a 2 mL volume and allowed to settle 1 h. For each construct to be transfected, 4 ul FuGENE 6 was added to 100 μl Sf900-II serum free media (SFM) (Thermo Fisher Scientific) and incubated 5 minutes at room temperature.

Expressing:

Article Title: Production of recombinant coiled coil silk proteins for materials synthesis.
Article Snippet: .. Virus generation and amplification for insect cell expression Passage1 (P1) virus was generated by transfecting Sf9 cells in a 6-well plate with 1 μg bacmid using FuGENE 6 (Promega) transfection reagent, following manufacturer’s instructions. .. Briefly, SF900-II suspension-adapted Sf9 in log growth phase were plated at a cell density of 1 × 106 cells/well in a 2 mL volume and allowed to settle 1 h. For each construct to be transfected, 4 ul FuGENE 6 was added to 100 μl Sf900-II serum free media (SFM) (Thermo Fisher Scientific) and incubated 5 minutes at room temperature.

Generated:

Article Title: Production of recombinant coiled coil silk proteins for materials synthesis.
Article Snippet: .. Virus generation and amplification for insect cell expression Passage1 (P1) virus was generated by transfecting Sf9 cells in a 6-well plate with 1 μg bacmid using FuGENE 6 (Promega) transfection reagent, following manufacturer’s instructions. .. Briefly, SF900-II suspension-adapted Sf9 in log growth phase were plated at a cell density of 1 × 106 cells/well in a 2 mL volume and allowed to settle 1 h. For each construct to be transfected, 4 ul FuGENE 6 was added to 100 μl Sf900-II serum free media (SFM) (Thermo Fisher Scientific) and incubated 5 minutes at room temperature.

Transfection:

Article Title: Production of recombinant coiled coil silk proteins for materials synthesis.
Article Snippet: .. Virus generation and amplification for insect cell expression Passage1 (P1) virus was generated by transfecting Sf9 cells in a 6-well plate with 1 μg bacmid using FuGENE 6 (Promega) transfection reagent, following manufacturer’s instructions. .. Briefly, SF900-II suspension-adapted Sf9 in log growth phase were plated at a cell density of 1 × 106 cells/well in a 2 mL volume and allowed to settle 1 h. For each construct to be transfected, 4 ul FuGENE 6 was added to 100 μl Sf900-II serum free media (SFM) (Thermo Fisher Scientific) and incubated 5 minutes at room temperature.



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